HYALURONAN ANALYSIS SERVICES

Our lab has developed processes for the extraction and measurement of hyaluronan from diverse biological matrices. We are now offering these capabilities as a fee-for-service to other labs interested in incorporating them into their own work. Our trained personnel can conduct a range of analyses as outlined below to fit your project needs. Contact us to arrange a consultation or fill out a quick survey to get started.

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Size Distribution

Using state-of-the-art solid-state nanopore technology, we are able to determine quantitative hyaluronan (HA) molecular weight distribution from as little as 10 ng of total material. From any purified HA sample, we can provide you with distribution data across a dynamic range from as little as 50 kDa to at least 20 MDa. You will receive raw data on HA molecular weight on a molecule-by-molecule basis as well as distribution data with basic statistical analysis. We can provide more information on HA isolation standards or you can use our in-house extraction services on your specimen.

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Extraction

We offer HA extraction services from a broad range of biological matrices, including plasma, urine, synovial fluid, and more. Our techniques provide an unbiased isolate that is representative of the total HA in a sample and is viable for size distribution analysis.

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Quantification

We use an ELISA-like assay to provide you HA quantification, performing all necessary pre-treatments based on the needs of your sample.

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Size distributions obtained for enzymatically-generated HA for increasing time courses. Sizes range up to 20 MDa. From Nat Comm, 10, 5527, 2019.

Size Distribution Analysis

Unbiased molecular weight distribution
The biophysics of our technology ensures that there is no bias towards any hyaluronan size over any other, resulting in an accurate representation of the distribution in your sample

 

Wide dynamic range
Nanopore analysis is capable of resolving molecular weights below 50 kDa and up to at least 20 MDa

 

High sensitivity
Common techniques like gel electrophoresis typically require micrograms of material to perform, creating challenges for many matrices that may have limited hyaluronan content; we can produce a full size distributions from as little as 10 ng hyaluronan total
 

 
 
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Hyaluronan extraction

High specificity

We strip binding entities and use hyaladherin proteins to capture hyaluronan from solution, leaving all other molecules behind and enabling a highly pure measurement

 

High fidelity

Our isolation protocols have been optimized to produce a size distribution that is faithful to that of the original matrix

 

High sensitivity

We are able to capture trace amounts of hyaluronan, maximizing analytical capability for your sample of interest

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Hyaluronan quantification

High sensitivity

We use the most reliable, highest sensitivity kit on the market, offering a resolution of 25 ng/mL

 

No infrastructure or personnel cost

Are you unfamiliar with ELISA-like assays? Do you not have access to necessary instrumentation? We can perform your analyses.