HYALURONAN ANALYSIS SERVICES
Using state-of-the-art solid-state nanopore technology, we are able to determine quantitative hyaluronan (HA) molecular weight distribution from as little as 10 ng of total material. From any purified HA sample, we can provide you with distribution data across a dynamic range from as little as 50 kDa to at least 20 MDa. You will receive raw data on HA molecular weight on a molecule-by-molecule basis as well as distribution data with basic statistical analysis. We can provide more information on HA isolation standards or you can use our in-house extraction services on your specimen.
We offer HA extraction services from a broad range of biological matrices, including plasma, urine, synovial fluid, and more. Our techniques provide an unbiased isolate that is representative of the total HA in a sample and is viable for size distribution analysis.
The total amount of HA present in a specimen can be critical, both as an independent metric and in combination with size distribution. We use an ELISA-like assay to provide quantification of HA, performing all necessary pre-treatments based on the needs of your sample.
Our lab has developed processes for the extraction and measurement of hyaluronan from diverse biological matrices. We are now offering these capabilities as a fee-for-service to other labs interested in incorporating them into their own work. Our trained personnel can conduct a range of analyses as outlined below to fit your project needs. To get started, you can contact us to arrange a consultation or jump down to see our current pricing.
Size distributions obtained for enzymatically-generated HA for increasing time courses. Sizes range up to 20 MDa. From Nat Comm, 10, 5527, 2019.
Size Distribution Analysis
Unbiased molecular weight distribution
The biophysics of our technology ensures that there is no bias towards any hyaluronan size over any other, resulting in an accurate representation of the distribution in your sample
Wide dynamic range
Nanopore analysis is capable of resolving molecular weights below 50 kDa and up to at least 20 MDa
Common techniques like gel electrophoresis typically require micrograms of material to perform, creating challenges for many matrices that may have limited hyaluronan content; we can produce a full size distributions from as little as 10 ng hyaluronan total
We strip binding entities and use hyaladherin proteins to capture hyaluronan from solution, leaving all other molecules behind and enabling a highly pure measurement
Our isolation protocols have been optimized to produce a size distribution that is faithful to that of the original matrix
We are able to capture trace amounts of hyaluronan, maximizing analytical capability for your sample of interest
We use the most reliable, highest sensitivity kit on the market, offering a resolution of 25 ng/mL
No infrastructure or personnel cost
Are you unfamiliar with ELISA-like assays? Do you not have access to necessary instrumentation? We can perform your analyses.
Below is a table of our current pricing*. Please contact us to find out if your particular samples require any special processing. All services are provided through iLab. After determining which services are appropriate with our team, you can initiate the service request form here using your own institutional login, providing your name and funding mechanism, and entering required fields. If you have any questions, encounter any trouble accessing iLab, or your institution does not use iLab, please contact us to discuss other options.
*Note that pricing covers personnel and cost of materials + reagents, plus required overhead for external parties.
(Internal to Wake Forest)
(External to Wake Forest)
Interfering materials (DMSO, etc.)
HA Molecular Weight Analysis
Single run (10 ng HA min.)
This service is provided as a part of the NIH-funded (P41EB020594) Center of BioModular Multi-Scale Systems for Precision Medicine, a National Biotechnology Resource Center.